The molecular structure of green fluorescent protein pdf. Fluorescent proteins and their applications in imaging living cells. Abstract we have investigatedproperties relevant to quantitative imaging in living cells of five green fluorescent protein. Recently, the use of inherently fluorescent proteins, such as green fluorescent protein gfp, has been described as a means to assess gene expression and. The green fluorescent protein gfp of the jellyfish aequorea victoria is a useful reporter molecule which requires neither substrates nor cofactors due to the intrinsically fluorescent nature of. The green fluorescent protein gfp is a protein composed of 238 amino acid residues 26. Green fluorescent protein gfp from the jellyfish aequorea victoria and its homologs from diverse marine animals are widely used as universal. Green fluorescent protein pioneers share 2008 nobel prize.
Green fluorescent protein what is this thing youtube. Quantitation of green fluorescent protein in microplates using the. Gfp and egfp enhanced gfp will be detected in fl1 when using the standard c6 filter configuration fl1 53030 bp. What is this thing trying to document grad school one youtube video at a time, from lab equipment to genetics lessons to interviews with other students. Therefore, we adapted a live, longterm tagging system to visualize a model listeria protein by fluorescence microscopy on a singlecell level in. Green fluorescent protein makes for living lasers a single mammalian cell expressing a mutant green fluorescent protein is the first living object to.
742 850 681 641 86 1424 158 550 116 330 760 613 89 1490 549 1332 1204 926 768 646 1093 1255 597 518 565 1090 1065 409 34